NOTE: This can only be done within iTunes  ‘Tested in iTunes 10’

  1. Plugin your iPhone
  2. Open iTunes
  3. Under DEVICES click on your iPhone DEVICES->iPhone
  4. At the Top Menu select Summary iPhone Summary
  5. On the open page under Options check the checkbox next to Manually manage music and videos iPhone Options
  6. On the right menu under DEVICES expand the iPhone device by clicking on the arrow and click once on Music iPhone Music
  7. A list of your songs will appear on the main screen. Right click on the song that you wish to delete and select Delete and the selected song will be deleted from your iPhone. Delete single song from iPhone